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Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
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Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
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December 19, 2014
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In this article, a protocol for infection of macrophages with Cryptococcus neoformans is described. Also, a method for sterol depletion from the macrophages is explained. These protocols provide a guide to study fungal infections in vitro and examine the role of sterols in such infections.
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Bryan, A. M., Farnoud, A. M., Mor, V., Del Poeta, M. Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans. J. Vis. Exp. (94), e52432, doi:10.3791/52432 (2014).
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