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Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care
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Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care
Bölümler
- 00:05Başlık
- 01:59Administration of Temozolomide and Whole Brain Irradiation to Tumor-bearing Mice (days 0-4)
- 03:26Cell Transfection, Splenectomy, and T Cell Preparation (day 0)
- 06:09Transduction (days 1 and 2)
- 08:10CAR T Cell and Administration to Tumor-bearing Mice (days 3-5)
- 10:03Results: Antitumor Efficacy of CAR T Cells
- 11:47Conclusion
The lymphodepletive and immunomodulatory effects of chemotherapy and radiation standard of care can be leveraged to enhance the antitumor efficacy of T cell immunotherapy. We outline a method for generating EGFRvIII-specific chimeric antigen receptor (CAR) T cells and administering them in the context of glioblastoma standard of care.
