Although researchers are generally knowledgeable about procedures and safety precautions required for biosafety level 1 or 2 (BSL-1/2) experiments, they may not be familiar with experimental procedures in BSL-4 suit laboratories. This article provides a detailed visual demonstration of BSL-4 suit laboratory systems check, laboratory entry, movement, and exit procedures.
Janosko, K., Holbrook, M. R., Adams, R., Barr, J., Bollinger, L., Newton, J. T., Ntiforo, C., Coe, L., Wada, J., Pusl, D., Jahrling, P. B., Kuhn, J. H., Lackemeyer, M. G. Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 1. Biosafety Level 4 Suit Laboratory Suite Entry and Exit Procedures. J. Vis. Exp. (116), e52317, doi:10.3791/52317 (2016).