Dergi
/
Biyomühendislik
/
样品制备策略的3D细胞培养模型质谱成像
JoVE Journal
Biyomühendislik
This content is Free Access.
JoVE Journal Biyomühendislik
Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
Please note that all translations are automatically generated. Click here for the English version.

样品制备策略的3D细胞培养模型质谱成像

17,742 Views

•

08:14 min

•

December 05, 2014

DOI:

10.3791/52313-v

DOI:
10.3791/52313-v

•

08:14 min
December 05, 2014

•

15 Views
, ,
1Department of Chemistry and Biochemistry,Notre Dame Üniversitesi, 2Harper Cancer Research Institute,Notre Dame Üniversitesi

Özet

Automatically generated

永生化癌细胞系可以种植三维细胞培养物，生物研究的一个有价值的模型。本协议描述的3D细胞培养，包括改进的样品制备平台质谱成像。该协议的目的是为了指导用户以制备三维细胞培养物用于质谱成像分析。

Explore More Videos

3D Cell CultureMass Spectrometry ImagingMALDI-MSISample PreparationColon CancerSpheroidsTumor NodulesSpatial AnalysisMolecular Distribution

Read Article

Cite this Article

Ahlf Wheatcraft, D. R., Liu, X., Hummon, A. B. Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models. J. Vis. Exp. (94), e52313, doi:10.3791/52313 (2014).

Download .ris file

Copy

Share Video

.

Copy