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纤毛对小鼠的主要嗅觉系统整装标签
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JoVE Journal Nörobilim
Whole Mount Labeling of Cilia in the Main Olfactory System of Mice
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纤毛对小鼠的主要嗅觉系统整装标签

10,885 Views

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08:42 min

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December 27, 2014

DOI:

10.3791/52299-v

DOI:
10.3791/52299-v

•

08:42 min
December 27, 2014

•

22 Views
,
1Pharmacology and Toxicology,Universitaetsklinikum Jena, 2Charite-Universitaetsmedizin Berlin

Özet

Automatically generated

Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.

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Whole Mount LabelingCiliaMain Olfactory SystemMiceOlfactory Sensory NeuronsDendritic KnobOlfactory Signal TransductionKirrel2Axon GuidanceAdhesion MoleculeActivity-dependent Localization

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Cite this Article

Oberland, S., Neuhaus, E. M. Whole Mount Labeling of Cilia in the Main Olfactory System of Mice. J. Vis. Exp. (94), e52299, doi:10.3791/52299 (2014).

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