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Whole Mount Labeling of Cilia in the Main Olfactory System of Mice
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JoVE Journal Nörobilim
Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

DOI:
10.3791/52299-v

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08:42 min

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December 27, 2014

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,
1Pharmacology and Toxicology,Universitaetsklinikum Jena, 2Charite-Universitaetsmedizin Berlin

Bölümler

  • 00:05Başlık
  • 01:24Preparing the Nasal Septum
  • 03:12Isolating the Olfactory Epithelium
  • 05:01Staininig the Epithelium
  • 06:53Results: Images of Olfactory Epithelium
  • 08:04Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.

Etiketler

Whole Mount LabelingCiliaMain Olfactory SystemMiceOlfactory Sensory NeuronsDendritic KnobOlfactory Signal TransductionKirrel2Axon GuidanceAdhesion MoleculeActivity-dependent Localization

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