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PeakForce定量的ナノメカニカルプロパティマッピングを使用した赤色光光受容体の原子間力顕微鏡
JoVE Journal
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JoVE Journal Mühendislik
Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
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PeakForce定量的ナノメカニカルプロパティマッピングを使用した赤色光光受容体の原子間力顕微鏡

11,548 Views

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14:13 min

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October 24, 2014

DOI:

10.3791/52164-v

DOI:
10.3791/52164-v

•

14:13 min
October 24, 2014

•

4 Views
, , , , ,
1Biyoloji Bölümü,Northeastern Illinois University, 2Kimya Bölümü,Northeastern Illinois University

Özet

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A method for investigating the structure of a protein photoreceptor using atomic force microscopy (AFM) is described in this paper. PeakForce Quantitative Nanomechanical Property Mapping (PF-QNM) reveals intact protein dimers on a mica surface.

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Atomic Force MicroscopyAFMPeakForce Quantitative Nanomechanical Property MappingPF-QNMRed-Light PhotoreceptorRpBphP3P3Rhodopseudomonas PalustrisBiological MacromoleculesSoft Materials

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Cite this Article

Kroeger, M. E., Sorenson, B. A., Thomas, J. S., Stojković, E. A., Tsonchev, S., Nicholson, K. T. Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping. J. Vis. Exp. (92), e52164, doi:10.3791/52164 (2014).

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