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Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
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JoVE Journal Mühendislik
Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

DOI:
10.3791/52164-v

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14:13 min

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October 24, 2014

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, , , , ,
1Biyoloji Bölümü,Northeastern Illinois University, 2Kimya Bölümü,Northeastern Illinois University

Bölümler

  • 00:05Başlık
  • 02:27Computer and Microscope Setup
  • 03:45Fluid Cell Assembly and Imaging of Mica Surface
  • 08:25Protein Deposition onto the Mica
  • 10:08Imaging Proteins on Mica
  • 12:20Results: AFM Images of Photoreceptor P3
  • 13:14Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

A method for investigating the structure of a protein photoreceptor using atomic force microscopy (AFM) is described in this paper. PeakForce Quantitative Nanomechanical Property Mapping (PF-QNM) reveals intact protein dimers on a mica surface.

Etiketler

Atomic Force MicroscopyAFMPeakForce Quantitative Nanomechanical Property MappingPF-QNMRed-Light PhotoreceptorRpBphP3P3Rhodopseudomonas PalustrisBiological MacromoleculesSoft Materials

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