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겐타 마이신 유도 부상으로 성인 Zebrafish의에서 신장 재생
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
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겐타 마이신 유도 부상으로 성인 Zebrafish의에서 신장 재생

10,621 Views

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06:25 min

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August 03, 2015

DOI:

10.3791/51912-v

DOI:
10.3791/51912-v

•

06:25 min
August 03, 2015

•

9 Views
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1Nephrology Division, Department of Medicine,Massachusetts General Hospital, 2Basic Sciences Division,Fred Hutchinson Cancer Research Center, 3Genetik Bölümü,Harvard Tıp Fakültesi

Özet

Automatically generated

Here we present a reliable method to study adult kidney regeneration by inducing acute kidney injury by gentamicin injection. We show that injury is dependent on gentamicin dosage and environmental temperature using in situ hybridization to label lhx1a+ developing new nephrons.

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Cite this Article

Kamei, C. N., Liu, Y., Drummond, I. A. Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury. J. Vis. Exp. (102), e51912, doi:10.3791/51912 (2015).

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