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Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

10,628 Views

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06:25 min

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August 03, 2015

DOI:

10.3791/51912-v

DOI:
10.3791/51912-v

•

06:25 min
August 03, 2015

•

9 Views
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1Nephrology Division, Department of Medicine,Massachusetts General Hospital, 2Basic Sciences Division,Fred Hutchinson Cancer Research Center, 3Department of Genetics,Harvard Medical School

Summary

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Here we present a reliable method to study adult kidney regeneration by inducing acute kidney injury by gentamicin injection. We show that injury is dependent on gentamicin dosage and environmental temperature using in situ hybridization to label lhx1a+ developing new nephrons.

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Cite this Article

Kamei, C. N., Liu, Y., Drummond, I. A. Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury. J. Vis. Exp. (102), e51912, doi:10.3791/51912 (2015).

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