Dergi
/
Nörobilim
/
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

DOI:
10.3791/51737-v

•

06:40 min

•

September 15, 2014

•

, ,
1Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 2Department of Obstetrics and Gynecology,Stanford University School of Medicine

Bölümler

  • 00:05Başlık
  • 01:11Preparation of Cells for Differentiation
  • 03:00Cell Differentiation
  • 04:39Results: Representative Dopaminergic Neuron Images
  • 06:13Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We, based on knowledge from developmental biology and published research, developed an optimized protocol to efficiently generate A9 midbrain dopaminergic neurons from both human embryonic stem cells and human induced pluripotent stem cells, which would be useful for disease modeling and cell replacement therapy for Parkinson’s disease.

Etiketler

Dopaminergic NeuronsPluripotent Stem CellsParkinson’s DiseaseCell Replacement TherapyFloor PlateVentral MidlineNeural DevelopmentDifferentiationHuman Embryonic Stem CellsHuman Induced Pluripotent Stem CellsIn Vitro Disease ModelingDrug ScreeningCell Transplantation

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Quantifying Levels of Dopaminergic Neuron Morphological Alteration and Degeneration in Caenorhabditis elegans

Read Article