Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses

This article provides a protocol for the extraction of venom from spiders using electrical stimulation in order to 1) conduct proteomic characterization, 2) stimulate venom gland gene expression, and 3) perform functional studies of venoms. This is followed by a description of venom gland microdissections for gene expression studies.