'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.' 'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
'Bölümler'
- 00:05'Başlık'
- 00:56Surgery
- 01:46Thinned-skull Preparation
- 03:27Immobilization
- 04:45Imaging
- 06:33Recovery
- 07:14Re-imaging
- 08:22Results: Transcranial Two-Photon Imaging Showing Dynamics of Dendritic Spines
- 09:31Conclusion
Time-lapse imaging in the living animal provides valuable information on structural reorganization in the intact brain. Here, we introduce a thinned-skull preparation that allows transcranial imaging of fluorescently labeled synaptic structures in the living mouse cortex by two-photon microscopy.
