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Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
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October 13, 2014
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1Department of Neurobiology,University of California, Los Angeles, 2Veterans Administration Greater Los Angeles Healthcare System, 3Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences,Dalhousie University, 4Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine,University of California, Los Angeles
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Immunohistochemistry protocols are used to study the localization of a specific protein in the retina. Calcium imaging techniques are employed to study calcium dynamics in retinal ganglion cells and their axons.
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Sargoy, A., Barnes, S., Brecha, N. C., Pérez De Sevilla Müller, L. Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina. J. Vis. Exp. (92), e51396, doi:10.3791/51396 (2014).
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