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Imagerie Effacé systèmes biologiques intacts au niveau cellulaire par 3DISCO
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Imagerie Effacé systèmes biologiques intacts au niveau cellulaire par 3DISCO

DOI:

07:49 min

July 07, 2014

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Bölümler

  • 00:05Başlık
  • 01:32Imaging Cleared Organs
  • 04:14Results: Representative 3DISCO Images of Cleared Tissues
  • 06:54Conclusion

Özet

Otomatik Çeviri

To obtain high-resolution images of fluorescently labeled cells within large tissues, ideally, the biological samples should be imaged without sectioning. 3DISCO is a straightforward tissue clearing procedure based on sequential incubation with organic solvents. Upon clearing, the organs become transparent allowing an end-to-end laser scan of the specimen.

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