We previously developed a technique for implanting tetrode wires into the central complex of cockroach brains that allows us to monitor activity in individual units of tethered cockroaches. Here we present a modified version of that technique that allows us to also record brain activity in freely moving insects.
Guo, P., Pollack, A. J., Varga, A. G., Martin, J. P., Ritzmann, R. E. Extracellular Wire Tetrode Recording in Brain of Freely Walking Insects. J. Vis. Exp. (86), e51337, doi:10.3791/51337 (2014).