'Dergi'
/
Nörobilim
/
Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
JoVE Journal
Nörobilim
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Nörobilim
Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

DOI:
10.3791/51298-v

•

09:25 min

•

June 04, 2014

•

,
1Department of Molecular Genetics and Microbiology,Duke University Medical Center, 2Departments of Neurobiology and Cell Biology, Duke Institute for Brain Sciences,Duke University Medical Center

'Bölümler'

  • 00:05'Başlık'
  • 01:17Dissection and Embedding of the Embryonic Brains
  • 02:24Embedded Brain Sectioning and Staining
  • 03:59Live Imaging of the Slices
  • 04:55Post-acquisition Analysis of Mitosis
  • 07:34Results: Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
  • 08:43Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Neural progenitor mitosis is a critical parameter of neurogenesis. Much of our understanding of neural progenitor mitosis is based on analysis of fixed tissue. Live imaging in embryonic brain slices is a versatile technique to assess mitosis with high temporal and spatial resolution in a controlled environment.

'Etiketler'

MitosisLive ImagingMouse Embryonic CortexNeural ProgenitorsBrain DevelopmentCerebral CortexTime-lapse ImagingSyto11Histone H2B-EGFPCentrin-EGFPMCherry-α-tubulinGenetic Regulation

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium

Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Read Article