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An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans
JoVE Journal
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An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans

An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans

9,696 Views

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09:24 min

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March 11, 2014

DOI:

10.3791/51231-v

DOI:
10.3791/51231-v

•

09:24 min
March 11, 2014

•

5 Views
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1Departamento de Bioquímica, Instituto de Química,Universidade de São Paulo, 2Department of Medicine, Nephrology Division,University of Rochester Medical Center, School of Medicine and Dentistry

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A protocol is described that uses anoxia/starvation in C. elegans to model ischemia/reperfusion. Functional outcomes include increased mortality, visible abnormalities in GFP-labeled neuronal processes, and impaired behavioral responses that require neuronal function.

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Queliconi, B. B., Kowaltowski, A. J., Nehrke, K. An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans. J. Vis. Exp. (85), e51231, doi:10.3791/51231 (2014).

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