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Um rato, duas culturas: isolamento e cultivo de células-tronco neurais Adulto de as duas zonas neurogênica Individual Ratos
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

Um rato, duas culturas: isolamento e cultivo de células-tronco neurais Adulto de as duas zonas neurogênica Individual Ratos

36,831 Views

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09:52 min

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February 25, 2014

DOI:

10.3791/51225-v

DOI:
10.3791/51225-v

•

09:52 min
February 25, 2014

•

30 Views
,
1Center for Regenerative Therapies Dresden,Technische Universität Dresden, 2German Center for Neurodegenerative Diseases (DZNE) Dresden

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Aqui, descrevemos um protocolo detalhado para a produção simultânea de culturas de células precursoras neurais, quer como monocamadas aderentes ou neuroesferas, a partir da zona subventricular e giro dentado de ratinhos adultos individuais.

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Walker, T. L., Kempermann, G. One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice. J. Vis. Exp. (84), e51225, doi:10.3791/51225 (2014).

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