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神经科学
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One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

DOI:
10.3791/51225-v

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09:52 min

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February 25, 2014

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1Center for Regenerative Therapies Dresden,Technische Universität Dresden, 2German Center for Neurodegenerative Diseases (DZNE) Dresden

Chapters

  • 00:05Title
  • 01:41Microdissection of Mouse Brain
  • 04:13SVZ Tissue Dissociation
  • 05:43DG Tissue Dissociation
  • 07:36Cell Plating for Monolayer and Neurosphere Culture Generation
  • 08:17Results: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
  • 09:21Conclusion

Summary

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Here we describe a detailed protocol for the simultaneous generation of neural precursor cell cultures, as either adherent monolayers or neurospheres, from the subventricular zone and dentate gyrus of individual adult mice.

Tags

Neural Stem CellsNeurosphere AssayAdherent Monolayer CultureAdult NeurogenesisSubventricular ZoneDentate GyrusCell ProliferationCell DifferentiationMouse Models

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