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'Bölümler'
- 00:04'Başlık'
- 02:48Growing Nicotiana Plants
- 03:55Vacuum Infiltration of Agrobacterium into Nicotiana Plants
- 07:41Western Blot Analysis
- 09:40GFP Imaging
- 10:21Results: Agrobacterium tumefaciens Infiltration is an Effective Method for Transient Protein Production in Nicotiana Species
- 22:19Conclusion
Transient protein production in Nicotiana plants based on vacuum infiltration with Agrobacteria carrying launch vectors (Tobacco mosaic virus-based) is a rapid and economic approach to produce vaccine antigens and therapeutic proteins. We simplified the procedure and improved target accumulation by optimizing conditions of bacteria cultivation, selecting host species, and co-introducing RNA silencing suppressors.
