Dergi
/
Tıp
/
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
JoVE Journal
Tıp
This content is Free Access.
JoVE Journal Tıp
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

22,830 Views

•

07:13 min

•

March 26, 2014

DOI:

10.3791/51171-v

DOI:
10.3791/51171-v

•

07:13 min
March 26, 2014

•

4 Views
, , , , , , , , , ,
1Tumor Microenvironment and Metastasis Program,Wistar Institute, 2Department of Pathology and Lab Medicine, Perelman School of Medicine,University of Pennsylvania, 3Department of Microbiology and Immunology and Department of Genetics,Geisel School of Medicine at Dartmouth, 4Division of Endocrine and Oncologic Surgery, Department of Surgery, Perelman School of Medicine,University of Pennsylvania, 5Rena Rowan Breast Center, Abramson Cancer Center,University of Pennsylvania, 6Center for Advanced Medicine,University of Pennsylvania

Özet

Automatically generated

Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.

Explore More Videos

Metastatic Breast CarcinomaTransgenic Mouse ModelDuctal EpitheliumAdenovirus-creP53 AblationK-ras OncogeneTumor ProgressionLymphovascular InvasionLeukocyte InfiltrationLatent MetastasisDormancyImmunotherapy

Read Article

Cite this Article

Rutkowski, M. R., Allegrezza, M. J., Svoronos, N., Tesone, A. J., Stephen, T. L., Perales-Puchalt, A., Nguyen, J., Zhang, P. J., Fiering, S. N., Tchou, J., Conejo-Garcia, J. R. Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer. J. Vis. Exp. (85), e51171, doi:10.3791/51171 (2014).

Download .ris file

Copy

Share Video

.

Copy