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Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

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10:14 min

December 17, 2013

DOI:

10:14 min
December 17, 2013

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Özet

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This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.

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