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The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
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The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

10,294 Views

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09:22 min

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December 13, 2013

DOI:

10.3791/50867-v

DOI:
10.3791/50867-v

•

09:22 min
December 13, 2013

•

6 Views
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1Department of Nephrology,Children’s Hospital of Pittsburgh of UPMC, 2Department of Cell Biology and Physiology,University of Pittsburgh School of Medicine

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Membrane trafficking involves transport of proteins from the plasma membrane to the cell interior (i.e. endocytosis) followed by trafficking to lysosomes for degradation or to the plasma membrane for recycling. Methods described in this article are designed to study endocytosis and recycling of plasma membrane proteins.

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Cite this Article

Cihil, K. M., Swiatecka-Urban, A. The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins. J. Vis. Exp. (82), e50867, doi:10.3791/50867 (2013).

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