/
/
??????????? ??? ??? ??????????? ??????????????? ??????? ????????? ??? ????? I ?? V ? ??????????-??????????<em> ?????????? ???????????</em
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
??????????? ??? ??? ??????????? ??????????????? ??????? ????????? ??? ????? I ?? V ? ??????????-??????????<em> ?????????? ???????????</em
1Centre for Antimicrobial Resistance,Alberta Health Services / Calgary Laboratory Services / University of Calgary, 2Department of Pathology & Laboratory Medicine,University of Calgary, 3Department of Microbiology, Immunology and Infectious Diseases,University of Calgary, 4Tıp Fakültesi,University of Calgary, 5The Calvin, Phoebe and Joan Snyder Institute for Chronic Diseases,University of Calgary
Bölümler
- 00:05Başlık
- 01:16Sample Preparation
- 02:30Polymerase Chain Reaction (PCR)
- 03:36Agarose Gel Electrophoresis
- 04:58PCR Products for Representative Strains of SCCmec
- 08:27Conclusion
We demonstrate a simple multiplex PCR assay for quick-screening and typing of Staphylococcal Cassette Chromosome mec (SCCmec) types I-V for methicillin-resistant Staphylococcus aureus, and provide some of the vital steps and procedural nuances that make it successful for adapting this assay to individual laboratories.
