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CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells
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Biyomühendislik
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CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

17,077 Views

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10:59 min

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October 18, 2014

DOI:

10.3791/50607-v

DOI:
10.3791/50607-v

•

10:59 min
October 18, 2014

•

6 Views
, , , , , , ,
1Department of Biological Engineering,Massachusetts Institute of Technology, 2Environmental Toxicology,Chulabhorn Graduate Institute, 3Department of Biomedical Engineering,University of Minnesota

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We describe here a platform that allows comet assay detection of DNA damage with unprecedented throughput. The device patterns mammalian cells into a microarray and enables parallel processing of 96 samples. The approach facilitates analysis of base level DNA damage, exposure-induced DNA damage and DNA repair kinetics.

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Ge, J., Prasongtanakij, S., Wood, D. K., Weingeist, D. M., Fessler, J., Navasummrit, P., Ruchirawat, M., Engelward, B. P. CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells. J. Vis. Exp. (92), e50607, doi:10.3791/50607 (2014).

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