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Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
JoVE Journal
Biyomühendislik
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JoVE Journal Biyomühendislik
Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

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14:09 min

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December 12, 2013

DOI:

10.3791/50566-v

DOI:
10.3791/50566-v

•

14:09 min
December 12, 2013

•

1 Views
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1Laboratoire Matière et Systèmes Complexes (MSC),CNRS/Université Paris Diderot, 2ImagoSeine BioImaging Core Facility, Institut Jacques Monod,CNRS/Université Paris Diderot, 3Laboratoire d’Immunopathologie et Chimie Thérapeutique,CNRS/Institut de Biologie Moléculaire et Cellulaire

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In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.

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Marangon, I., Boggetto, N., Ménard-Moyon, C., Luciani, N., Wilhelm, C., Bianco, A., Gazeau, F. Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry. J. Vis. Exp. (82), e50566, doi:10.3791/50566 (2013).

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