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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
JoVE Journal
Biyomühendislik
This content is Free Access.
JoVE Journal Biyomühendislik
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

DOI:

12:04 min

December 06, 2013

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Bölümler

  • 00:05Başlık
  • 02:05Wafer Fabrication
  • 04:17Polydimethylsiloxane Chip Fabrication
  • 06:50Experimental Setup
  • 08:34Seeding of Bacterial Cells and Time-lapse Imaging
  • 10:30Results: Quantification of Microcolony Growth
  • 11:26Conclusion

Özet

Otomatik Çeviri

In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.

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