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Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities
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JoVE Journal Nörobilim
Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities

Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities

20,789 Views

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08:10 min

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March 31, 2014

DOI:

10.3791/50557-v

DOI:
10.3791/50557-v

•

08:10 min
March 31, 2014

•

2 Views
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1Department of Molecular Physiology & Biophysics,University of Iowa Carver College of Medicine, 2Department of Biology & Biochemistry,University of Bath

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We describe the use of styryl FM dyes to image synaptic vesicle recycling in functional nerve terminals. This protocol can be applied not only to evoked, but also spontaneous and miniature synaptic activities. The protocol expands the variety of synaptic events that can be effectively evaluated.

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Iwabuchi, S., Kakazu, Y., Koh, J., Goodman, K. M., Harata, N. C. Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities. J. Vis. Exp. (85), e50557, doi:10.3791/50557 (2014).

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