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Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
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Nörobilim
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JoVE Journal Nörobilim
Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

19,009 Views

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05:48 min

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May 23, 2013

DOI:

10.3791/50415-v

DOI:
10.3791/50415-v

•

05:48 min
May 23, 2013

•

9 Views
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1DFG-Center for Regenerative Therapies Dresden,Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden

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In this article, we demonstrate a method for manipulation of gene expression in the ventricular cells of the adult zebrafish telencephalon using antisense morpholino oligonucleotides. We present this method as an efficient and quick protocol that can be used for functional studies in the adult vertebrate brain.

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Gene ExpressionAdult Zebrafish BrainCerebroventricular MicroinjectionMorpholino OligonucleotidesRadial Glial CellsNeurogenesisNeurodegenerationCentral Nervous System Regeneration

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Kizil, C., Iltzsche, A., Kaslin, J., Brand, M. Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides. J. Vis. Exp. (75), e50415, doi:10.3791/50415 (2013).

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