Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions
Bölümler
- 00:05Başlık
- 02:06Syngeneic Murine Bone Marrow Transplantation (BMT)
- 03:11Tumor Engraftment
- 04:01Multi-parameter Immunofluorescent Staining
- 06:13Multispectral Imaging
- 07:10Quantitative Analysis of Multispectral Images
- 08:00Sonuçlar
- 10:32Conclusion
Complex tissue masses, from organs to tumors, are composed of various cellular elements. We elucidated the contribution of cellular phenotypes within a tissue utilizing multi-labeled fluorescent transgenic mice in combination with multiparameter immunofluorescent staining followed by spectral unmixing to decipher cell origin as well as cell characteristics based on protein expression.
