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Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development
Bölümler
- 00:05Başlık
- 02:00Ex utero Electroporations with GFP
- 04:04Whole Hemisphere Explant Preparation
- 06:25Fixation, Embedding, and Sectioning
- 09:30Neuronal Development in Injected, Cultured, and Processed Hemispheres
- 13:11Conclusion
This protocol describes an improved explant procedure that involves ex utero electroporation, dissection and culture of entire cerebral hemispheres from the embryonic mouse. The preparation facilitates pharmacological studies and assays of gene function during early cortical development.
