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New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

DOI:

09:27 min

May 30, 2013

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Bölümler

  • 00:05Başlık
  • 01:44Regulatory T cell isolation from HIV-1 Positive Blood
  • 03:40Cell Culture
  • 04:27Phenotyping
  • 05:14Suppression Assay
  • 07:18Results: Representative Treg Cells Expanded from HIV-1-infected Individuals
  • 08:33Conclusion

Özet

Otomatik Çeviri

CD4+ Regulatory T cells are potent immune-modulators and serve important functions in immune homeostasis. The paucity of these cells in peripheral blood makes functional studies challenging, specifically in the context of HIV-1-infection. We here describe a method to isolate and expand functional CD4+ Tregs from peripheral blood from HIV-1-infected individuals.

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