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体外红血细胞溶血含量的生物大分子药物的细胞内交货的评价的pH响应Endosomolytic代理
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11:12 min
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March 09, 2013
DOI:
1Department of Biomedical Engineering,Vanderbilt University, 2Vanderbilt Institute for Nanoscale Science & Engineering,Vanderbilt University, 3Interdisciplinary Materials Science Program,Vanderbilt University, 4Monroe Carell Jr. Children’s Hospital,Vanderbilt University Medical Center, 5Department of Chemical & Biomolecular Engineering,Vanderbilt University, 6Department of Cancer Biology,Vanderbilt University
Özet
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溶血试验可作为一种快速，高通量筛选，药物传递系统的细胞相容性和内货物配送endosomolytic活动的。该试验测量作为环境pH值的函数的红细胞膜的破坏。
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Cite this Article
Evans, B. C., Nelson, C. E., Yu, S. S., Beavers, K. R., Kim, A. J., Li, H., Nelson, H. M., Giorgio, T. D., Duvall, C. L. Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs. J. Vis. Exp. (73), e50166, doi:10.3791/50166 (2013).
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