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Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
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Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
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11:12 min
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March 09, 2013
DOI:
1Department of Biomedical Engineering,Vanderbilt University, 2Vanderbilt Institute for Nanoscale Science & Engineering,Vanderbilt University, 3Interdisciplinary Materials Science Program,Vanderbilt University, 4Monroe Carell Jr. Children’s Hospital,Vanderbilt University Medical Center, 5Department of Chemical & Biomolecular Engineering,Vanderbilt University, 6Department of Cancer Biology,Vanderbilt University
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A hemolysis assay can be used as a rapid, high-throughput screen of drug delivery systems' cytocompatibility and endosomolytic activity for intracellular cargo delivery. The assay measures the disruption of erythrocyte membranes as a function of environmental pH.
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Evans, B. C., Nelson, C. E., Yu, S. S., Beavers, K. R., Kim, A. J., Li, H., Nelson, H. M., Giorgio, T. D., Duvall, C. L. Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs. J. Vis. Exp. (73), e50166, doi:10.3791/50166 (2013).
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