Infection of the prostate may be a contributing factor in mediating pelvic pain in chronic prostatitis. We describe the procedure for preparation of standardized bacterial inoculum, instillation of bacteria into the urethra of male mice and methodology for measuring tactile allodynia in mice over time.
Quick, M. L., Done, J. D., Thumbikat, P. Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis. J. Vis. Exp. (71), e50158, doi:10.3791/50158 (2013).