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Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
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Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

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08:44 min

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September 24, 2013

DOI:

10.3791/50146-v

DOI:
10.3791/50146-v

•

08:44 min
September 24, 2013

•

9 Views
, , , ,
1Department of Neuropathology,Medical School Düsseldorf, 2Department of Molecular Genetics,Weizmann Institute for Science, 3Center of Behavioral Neuroscience,University of Düsseldorf

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Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.

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Vomund, S., Sapir, T., Reiner, O., de Souza Silva, M. A., Korth, C. Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening. J. Vis. Exp. (79), e50146, doi:10.3791/50146 (2013).

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