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单细胞测量液泡破裂引起细胞内病原体
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
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单细胞测量液泡破裂引起细胞内病原体

13,282 Views

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10:39 min

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June 12, 2013

DOI:

10.3791/50116-v

DOI:
10.3791/50116-v

•

10:39 min
June 12, 2013

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1 Views
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1Dynamique des Interactions Hôte Pathogène,Institut Pasteur, Paris, France, 2Imagopole,Institut Pasteur, Paris, France, 3Pathogenomique Mycobacterienne Integrée,Institut Pasteur, Paris, France

Özet

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我们描述了一种用于跟踪内膜破裂引起的细胞内的细菌<em>痢疾杆菌<em></em</em>和<em<em><em>结核杆菌</em></em</em>侵入宿主细胞后。我们的检测利用CCF4，宿主细胞质FRET探针在活的或固定的细胞。记者降解的酶活性存在于细菌表面。

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Single CellVacuolar RuptureIntracellular PathogensShigella FlexneriMycobacterium TuberculosisCCF4 FRET ReporterPhagosomal Membrane DisruptionReal-time ImagingHigh-throughput Analysis

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Cite this Article

Keller, C., Mellouk, N., Danckaert, A., Simeone, R., Brosch, R., Enninga, J., Bobard, A. Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens. J. Vis. Exp. (76), e50116, doi:10.3791/50116 (2013).

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