Journal
/
免疫与感染
/
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

13,305 Views

•

10:39 min

•

June 12, 2013

DOI:

10.3791/50116-v

DOI:
10.3791/50116-v

•

10:39 min
June 12, 2013

•

1 Views
, , , , , ,
1Dynamique des Interactions Hôte Pathogène,Institut Pasteur, Paris, France, 2Imagopole,Institut Pasteur, Paris, France, 3Pathogenomique Mycobacterienne Integrée,Institut Pasteur, Paris, France

Summary

Automatically generated

We describe a method for tracking the endomembrane rupture elicited by the intracellular bacteria Shigella flexneri and Mycobacterium tuberculosis upon host cell invasion. Our assay makes use of CCF4, a host cytoplasmic FRET probe in live or fixed cells. This reporter is degraded by an enzyme activity present on the bacterial surface.

Explore More Videos

Single CellVacuolar RuptureIntracellular PathogensShigella FlexneriMycobacterium TuberculosisCCF4 FRET ReporterPhagosomal Membrane DisruptionReal-time ImagingHigh-throughput Analysis

Read Article

Cite this Article

Keller, C., Mellouk, N., Danckaert, A., Simeone, R., Brosch, R., Enninga, J., Bobard, A. Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens. J. Vis. Exp. (76), e50116, doi:10.3791/50116 (2013).

Download .ris file

Copy

Share Video

.

Copy