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单个肌纤维和成人骨骼肌卫星细胞的分离和培养
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
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单个肌纤维和成人骨骼肌卫星细胞的分离和培养

61,383 Views

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11:57 min

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March 22, 2013

DOI:

10.3791/50074-v

DOI:
10.3791/50074-v

•

11:57 min
March 22, 2013

•

42 Views
, ,
1Sprott Center for Stem Cell Research,Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine,University of Ottawa

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肌纤维的分离和培养是黄金标准<em在体外</em系统研究通过平静，激活和分化的过渡卫星细胞。重要的是，单个肌纤维的文化系统保留的肌纤维/干细胞协会，这是一个必不可少的组成部分的肌肉干细胞小生境。

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Cite this Article

Pasut, A., Jones, A. E., Rudnicki, M. A. Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle. J. Vis. Exp. (73), e50074, doi:10.3791/50074 (2013).

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