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Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
 

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

Article DOI: 10.3791/50074-v • 11:57 min • March 22nd, 2013
March 22nd, 2013 •
1,2, 1,2, 1,2
1Sprott Center for Stem Cell Research, Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine, University of Ottawa

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Isolation and culture of myofibers is the gold standard in vitro system to study the transition of satellite cells through quiescence, activation and differentiation. Importantly, the single myofiber culture system preserves the myofiber/stem cell association, which is an essential component of the muscle stem cell niche.

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Isolation Culture Individual Myofibers Satellite Cells Adult Skeletal Muscle Muscle Regeneration Resident Stem Cells Satellite Cells Behavior Single Myofiber Isolation Protocol Flexor Digitorum Brevis (FDB) In Vitro System Physical Association Collagenase Digestion Rosenblatt Protocol Bischoff Protocol Gravity Sedimentation Unique Advantages Basal Lamina Fluorescence Activated Cell Sorting Tissue Dissociation Myofiber Culture System
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