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Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
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Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation

Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation

13,426 Views

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11:39 min

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June 01, 2013

DOI:

10.3791/50007-v

DOI:
10.3791/50007-v

•

11:39 min
June 01, 2013

•

4 Views
,
1Department of Ophthalmology and Visual Sciences,University of Nebraska Medical Center , 2Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center

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We describe the preparation of thin retinal slices from aquatic tiger salamanders (Ambystoma tigrinum) and explain how we use these slices to study synaptic processing in the retina by obtaining dual whole-cell voltage clamp recordings from photoreceptors and second-order horizontal and bipolar cells.

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Retinal NeuroscienceRetinal CircuitryPhotoreceptorsRetinal NeuronsElectrophysiological RecordingVertical Retinal SliceDual Whole-cell Voltage ClampPhotoreceptor Membrane PotentialSynaptic RibbonNeurotransmitter Release

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Van Hook, M. J., Thoreson, W. B. Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation. J. Vis. Exp. (76), e50007, doi:10.3791/50007 (2013).

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