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Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium
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06:41 min
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April 30, 2013
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Özet
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Here we develop the tools necessary for ex vivo live imaging to trace single cell divisions in the mouse E8.5 neuroepithelium
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Ex Vivo Live ImagingSingle Cell DivisionsMouse NeuroepitheliumFluorescent MarkersCulturing SlicesEmbryonic Mouse NeuroepitheliumGenetic Cell Lineage TracingTamoxifen-inducible Cre LineCre Reporter LineDsRed ReporterCre-mediated RecombinationTamoxifen InductionIndividual Cell DivisionsTranscriptional ResponseSonic Hedgehog SignalingOlig2-eGFP Transgenic LineCilia MarkerSstr3-GFP ReporterNeuroepithelium ImagingTime-lapse Confocal ImagingPrimary Cilia FormationShh Response
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Piotrowska-Nitsche, K., Caspary, T. Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium. J. Vis. Exp. (74), e4439, doi:10.3791/4439 (2013).
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