Dergi
/
Nörobilim
/
Viral Tracing of Genetically Defined Neural Circuitry
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Viral Tracing of Genetically Defined Neural Circuitry

Viral Tracing of Genetically Defined Neural Circuitry

DOI:
10.3791/4253-v

•

13:06 min

•

October 17, 2012

•

,
1Genetik Bölümü,Harvard Tıp Fakültesi, 2Howard Hughes Medical Institute,Harvard Tıp Fakültesi

Bölümler

  • 00:05Başlık
  • 01:16Passage and Concentration of Vesicular Stomatitis Virus (VSV)
  • 04:23Stereotaxic Injection of Virus
  • 08:19Tissue Harvest and Preparation for Microscopic Analysis
  • 09:41Results: Behavior of rVSV(A/RG) Virus in Various Cell Types
  • 12:22Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

A method of tracing synaptically connected neurons is described. We use TVA specificity of an upstream cell to probe whether a cell population of interest receives synaptic input from genetically defined cell types.

Etiketler

Viral TracingGenetically Defined Neural CircuitryTranssynaptic VirusesPseudorabies VirusRabies VirusVesicular Stomatitis VirusAnterograde DirectionRetrograde DirectionMonosynaptic Retrograde TracingGlycoprotein GeneChimeric GTVA ReceptorInputs Onto A Defined Cell TypeVSV As A Transsynaptic Tracer

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Physiological, Morphological and Neurochemical Characterization of Neurons Modulated by Movement

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection

Selective Tracing of Auditory Fibers in the Avian Embryonic Vestibulocochlear Nerve

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

A Lateralized Odor Learning Model in Neonatal Rats for Dissecting Neural Circuitry Underpinning Memory Formation

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Laser-guided Neuronal Tracing In Brain Explants

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Read Article