This content is Free Access.
Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
17,652 Views
•
06:30 min
•
July 26, 2012
DOI:
Özet
Automatically generated
This article describes in detail a protocol to electroporate in utero the cerebral cortex and the hippocampus at E14.5 in mice. We also show that this is a valuable method to study dendrites and spines in these two cerebral regions.
Explore More Videos
VisualizationGenetic ManipulationDendritesSpinesMouse Cerebral CortexHippocampusIn Utero ElectroporationIUEDevelopmentNervous SystemCellular ProliferationNeuronal MigrationCerebral CortexHippocampusNeuronsPrimary CulturesDendrite DevelopmentSpine DevelopmentSynapse DevelopmentGolgi-Cox MethodFluorescent Constructs
Read Article
Cite this Article
Pacary, E., Haas, M. A., Wildner, H., Azzarelli, R., Bell, D. M., Abrous, D. N., Guillemot, F. Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation. J. Vis. Exp. (65), e4163, doi:10.3791/4163 (2012).
Copy