Dergi
/
Tıp
/
NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts
JoVE Journal
Tıp
This content is Free Access.
JoVE Journal Tıp
NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

DOI:
10.3791/4115-v

•

12:07 min

•

July 24, 2012

•

, , , ,
1Electrical and Computer Engineering Department,The George Washington University, 2Pharmacology and Physiology Department,The George Washington University

Bölümler

  • 00:05Başlık
  • 01:25Setting Up for the Study
  • 02:44Heart Excision
  • 03:50Biventricular Cannulation
  • 07:21Signal Acquisition: Pressures, Monophasic Action Potentials, and fNADH
  • 09:08Typical Pressure, Monophasic Action Potentials, and fNADH Data
  • 11:12Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The objective is to monitor the mitochondrial redox state of isolated hearts within the context of physiologic preload and afterload pressures. A biventricular working rabbit heart model is presented. High spatiotemporal resolution fluorescence imaging of NADH is used to monitor the mitochondrial redox state of epicardial tissue.

Etiketler

NADH Fluorescence ImagingIsolated Perfused HeartLangendorff TechniqueCardiac PhysiologyCardiac MetabolismLV Working Heart ModelBiventricular ModelStroke VolumeCardiac OutputPressure DevelopmentMyocardial InjuryIschemiaNADH Fluorescence

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

Intramyocardial Cell Delivery: Observations in Murine Hearts

An Isolated Working Heart System for Large Animal Models

Working with Human Tissues for Translational Cancer Research

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Semi-Minimal Invasive Method to Induce Myocardial Infarction in Rats and the Assessment of Cardiac Function by an Isolated Working Heart System

Biventricular Assessment of Cardiac Function and Pressure-Volume Loops by Closed-Chest Catheterization in Mice

Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts

Isolated Lung Perfusion System in the Rabbit Model

Read Article