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Preparation of Acute Subventricular Zone Slices for Calcium Imaging
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September 19, 2012
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A method to load subventricular zone (SVZ) cells with calcium indicator dyes for recording calcium activity is described. The postnatal SVZ contains tightly packed cells including neural progenitor cells and neuroblasts. Rather than using bath loading we injected the dye by pressure inside the tissue allowing better dye diffusion.
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Acute Subventricular ZoneSlicesCalcium ImagingNeurogenic ZoneSVZ AstrocytesNeuroblastsIntermediate Progenitor CellsInterneuronsAdhesion MoleculesDiffusible SignalsIntercellular Calcium ActivityFunctional Intercellular SignalingCalcium Indicator DyeBath LoadingCellular DensitySagittal SlicesThree-dimensional ArrangementNeuroblast Migration
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Lacar, B., Young, S. Z., Platel, J., Bordey, A. Preparation of Acute Subventricular Zone Slices for Calcium Imaging. J. Vis. Exp. (67), e4071, doi:10.3791/4071 (2012).
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