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Isolation of Immune Cells from Primary Tumors
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June 16, 2012
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In this report, we describe a protocol for isolating highly purified populations of leukocytes that infiltrate tumors. This protocol is adapted from the Miltenyi Biotech protocol to enhance yield and purity for isolating cells from complex tumor tissue.
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IsolationImmune CellsPrimary TumorsImmunosuppressive MicroenvironmentLeukocytesChemokinesGrowth FactorsTumor-associated LeukocytesTumor-draining Lymph NodesSpleenCell ActivationTraffickingDendritic CellsPhenotypeAdoptively-transferred T CellsTolerizedProliferationCytokine ProductionTumor MicroenvironmentCD4+ T Helper CellsCD8+ T Cells
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Watkins, S. K., Zhu, Z., Watkins, K. E., Hurwitz, A. A. Isolation of Immune Cells from Primary Tumors. J. Vis. Exp. (64), e3952, doi:10.3791/3952 (2012).
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