Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
13,683 Views
•
10:03 min
•
September 23, 2012
DOI:
Özet
Automatically generated
Multiphoton microscopy of whole mouse organs is possible by optically clearing the organ before imaging, but not all protocols preserve the fluorescent signal of fluorescent proteins. Using an optical clearing method with ethanol-based dehydration and benzyl alcohol:benzyl benzoate clearing, we show high-resolution multiphoton images of whole mouse brain expressing YFP.
Explore More Videos
Multiphoton MicroscopyCleared Mouse BrainYFP ExpressionIntrinsic FluorescenceSecond Harmonic Generation (SHG)Optically ClearingMethanol DehydrationBenzyl Alcohol:benzyl Benzoate (BABB)Fluorescent SignalOptical Clearing ProtocolsEthanol Graded SeriesFluorescent ProteinsMultiphoton ImagingHigh-resolution ImagesNeurons
Read Article
Cite this Article
Parra, S. G., Vesuna, S. S., Murray, T. A., Levene, M. J. Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP. J. Vis. Exp. (67), e3848, doi:10.3791/3848 (2012).
Copy