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Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

JoVE Journal
Biyomühendislik

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JoVE Journal Biyomühendislik

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

DOI:

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09:52 min

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November 25, 2011

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Christine M. O’Brien, Kyle Rood, Shramik Sengupta, Sagar K. Gupta, Thiago DeSouza, Aaron Cook, John A. Viator

¹Biological Engineering, Christopher S. Bond Life Sciences Center, Dermatology,University of Missouri

Bölümler

00:05Başlık
01:20Blood Sample Preparation
02:58Flow Chamber Construction
04:32Photoacoustic Flowmetry Set-up
06:42Immunocytochemistry
08:39Results: Melanoma Isolation
09:30Conclusion

Özet

Otomatik Çeviri

We have developed a flow cytometer using laser induced ultrasound to detect circulating melanoma cells as an early indicator of metastatic disease.

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